tk gene meaning in Chinese
胸苷激酶基因
Examples
- Tk gene mutation test
Tk基因突变试验 - The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein . upstream of the tk orf , three putative gc boxes are located at positions - 22 , - 166 and - 199 . a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306
在tkorf上游- 22 、 - 166 、 - 199位存在3个gc框样序列，在终止密码子下游第110个核苷酸处的1306位存在有多聚腺苷加尾信号。 - Three pairs of primers were designed according to the sequences pulished by the genebank in order to amplifiy gd , ge and tk gene of the pseudorabies virus min - a strain . the gd , ge and tk gene were obtained by polymerase chain reaction ( pcr ) , and then cloned into the pgem - t easy vector
参考genebank收录的伪狂犬病病毒gd 、 ge 、 tk基因的序列设计了三对引物，对prvmin - a株进行了pcr扩增，扩增产物克隆于pgem - teasy载体。 - In this study , a 1 . 7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ) . the new transfer vector was called puni - lacz . the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的经典弱毒疫苗bartha - k61株为亲本株，在通用prv转移载体pbdtk - uni的基础上，在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒，同时将下游同源臂增加了一个1 . 7kb的kpni片段，使上下游同源臂的长度都超过了1kb ，构建了一个新的转移载体puni - lacz 。 - Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 %
本研究中首次对iltv - nm98a株的tk基因进行了克隆和序列分析，结果表明： iltv - nm98a株tk基因的核苷酸序列与已发表的iltvtk基因的核苷酸序列具有高度的同源性，两者之间仅相差4个核苷酸，同源性高达99 . 7 ，从而证实了iltvtk基因是高度保守的，为iltvtk基因核酸探针的制备提供了有力的依据。